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Object[Data, Western]

Data from a capillary electrophoresis-based Western blotting experiment.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Western, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Western]
    Pattern Description: Object[Data, Western]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Antibody Labeling

    PrimaryIncubationTime

    The duration for which the capillary was incubated with the PrimaryAntibody.
    Format: Single
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterEqualP[0 Second]

Imaging

    LuminescenceReagent

    The solution that reacted with the PeroxidaseReagent or the horseradish peroxidase (HRP) attached to the SecondaryAntibody to give off chemiluminesence which was observed during the SignalDetectionTimes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample] | Model[Sample] | Object[Sample] | Model[Sample, StockSolution]

Experimental Results

    MassSpectrum

    Mass spectra recorded via luminescent visualization of the capillary post separation, fixing, blocking, and staining.
    Format: Single
    Class: QuantityArray
    Unit: ['1 kilogram per mole', '1 lumen']
    Programmatic Pattern: QuantityCoordinatesP[{Kilo Dalton, Lumen}]

Analysis & Reports

    DataType

    Indicates if this data object represents a standard ladder or an analyte sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: Analyte | Ladder

    LadderSizes

    The sizes of the fragments in the provided ladder.
    Format: Computable
    Programmatic Pattern: {GreaterEqualP[0 Nucleotide, 1 Nucleotide]..} | {GreaterEqualP[0 Molar, 1 Molar]..}
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Computables`Private`ladderSizes[Field[LadderAnalyses]]]

    LadderPeaks

    The positions of the peaks in the ladder standard.
    Format: Computable
    Programmatic Pattern: {(GreaterP[0 Second, 1 Second] | GreaterP[0 Pixel, 1 Pixel] | GreaterP[0 Meter, 1 Meter] -> GreaterP[0 Nucleotide] | GreaterP[0 Molar])..}
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Computables`Private`ladderPeaks[Field[LadderAnalyses]]]

    LadderAnalyses

    A link to the ladder analysis object for these data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    LadderData

    Data containing the ladder run alongside with this analyte.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, Western][Analytes]

    Analytes

    Relations pointing to all of the data run in closest proximity to this ladder.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, Western][LadderData]

    MassSpectrumPeaksAnalyses

    Peak-picking analysis conducted on these data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    SmoothingAnalyses

    Smoothing analysis performed on this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    FitSourceSpectra

    Fitting analysis conducted on this spectrum.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:44
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