Object[Data, NephelometryKinetics]
Measurements of the time course changes in intensity of scattered light generated by a nephelometer.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, NephelometryKinetics, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, NephelometryKinetics]
Pattern Description: Object[Data, NephelometryKinetics]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Replicates
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Replicates]
General
Protocol
The protocol that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Data] | Object[Protocol][EnvironmentalData] | Object[Protocol, AbsorbanceQuantification][VolumeData] | Object[Protocol, AbsorbanceQuantification][EmptyPlateAbsorbance] | Object[Protocol, AbsorbanceQuantification][BufferAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][BlankAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][InitialPurgePressure] | Object[Protocol, AbsorbanceSpectroscopy][PurgePressureLog] | Object[Protocol, AbsorbanceIntensity][BlankAbsorbance] | Object[Protocol, AbsorbanceIntensity][InitialPurgePressure] | Object[Protocol, AbsorbanceIntensity][PurgePressureLog] | Object[Protocol, AbsorbanceKinetics][BlankAbsorbance] | Object[Protocol, AcousticLiquidHandling][Data] | Object[Protocol, AlphaScreen][GainOptimizationData] | Object[Protocol, AlphaScreen][FocalHeightOptimizationData] | Object[Protocol, BioLayerInterferometry][Data] | Object[Protocol, CyclicVoltammetry][ElectrodePretreatmentData] | Object[Protocol, CyclicVoltammetry][CyclicVoltammetryData] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionData] | Object[Protocol, CyclicVoltammetry][PretreatmentSpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][SpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionSpargingPressureLogs] | Object[Protocol, CircularDichroism][BlankData] | Object[Protocol, CircularDichroism][EmptyData] | Object[Protocol, DNASynthesis][DeblockSolutionWeightData] | Object[Protocol, DNASynthesis][WashSolutionWeightData] | Object[Protocol, DNASynthesis][InitialPurgePressure] | Object[Protocol, DynamicFoamAnalysis][Data] | Object[Protocol, Evaporate][CurrentWasteWeightData] | Object[Protocol, Evaporate][WasteWeightData] | Object[Protocol, Evaporate][BathActualTemperature] | Object[Protocol, Evaporate][BucketWeights] | Object[Protocol, PAGE][LadderData] | Object[Protocol, AgaroseGelElectrophoresis][LadderData] | Object[Protocol, MassSpectrometry][MatrixData] | Object[Protocol, MassSpectrometry][CalibrantData] | Object[Protocol, MassSpectrometry][SystemPrimeData] | Object[Protocol, MassSpectrometry][SystemFlushData] | Object[Protocol, HPLC][StandardData] | Object[Protocol, HPLC][BlankData] | Object[Protocol, HPLC][FlushData] | Object[Protocol, HPLC][PrimeData] | Object[Protocol, HPLC][SystemFlushData] | Object[Protocol, HPLC][SystemPrimeData] | Object[Protocol, HPLC][WasteWeightData] | Object[Protocol, FPLC][StandardData] | Object[Protocol, FPLC][BlankData] | Object[Protocol, FPLC][WasteWeightData] | Object[Protocol, FPLC][PrimeData] | Object[Protocol, FPLC][FlushData] | Object[Protocol, FPLC][SystemPrimeData] | Object[Protocol, FPLC][SystemFlushData] | Object[Protocol, GasChromatography][StandardData] | Object[Protocol, GasChromatography][BlankData] | Object[Protocol, GasChromatography][InitialHeliumPressure] | Object[Protocol, GasChromatography][InitialMethanePressure] | Object[Protocol, GasChromatography][FinalHeliumPressure] | Object[Protocol, GasChromatography][FinalMethanePressure] | Object[Protocol, GasChromatography][InitialHydrogenGeneratorWaterWeight] | Object[Protocol, GasChromatography][FinalHydrogenGeneratorWaterWeight] | Object[Protocol, IonChromatography][StandardData] | Object[Protocol, IonChromatography][AnionStandardData] | Object[Protocol, IonChromatography][CationStandardData] | Object[Protocol, IonChromatography][BlankData] | Object[Protocol, IonChromatography][AnionBlankData] | Object[Protocol, IonChromatography][CationBlankData] | Object[Protocol, IonChromatography][FlushData] | Object[Protocol, IonChromatography][AnionFlushData] | Object[Protocol, IonChromatography][CationFlushData] | Object[Protocol, IonChromatography][PrimeData] | Object[Protocol, IonChromatography][AnionPrimeData] | Object[Protocol, IonChromatography][CationPrimeData] | Object[Protocol, IonChromatography][SystemFlushData] | Object[Protocol, IonChromatography][AnionSystemFlushData] | Object[Protocol, IonChromatography][CationSystemFlushData] | Object[Protocol, IonChromatography][SystemPrimeData] | Object[Protocol, IonChromatography][AnionSystemPrimeData] | Object[Protocol, IonChromatography][CationSystemPrimeData] | Object[Protocol, IonChromatography][WasteWeightData] | Object[Protocol, ImageSample][RulerImageData] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, PNASynthesis][WashSolutionWeightData] | Object[Protocol, PNASynthesis][PrimaryWasteWeightData] | Object[Protocol, PNASynthesis][SecondaryWasteWeightData] | Object[Protocol, PNASynthesis][PurgePressureData] | Object[Protocol, PNASynthesis][PurgePressureLog] | Object[Protocol, PNASynthesis][InitialPurgePressure] | Object[Protocol, PeptideSynthesis][WashSolutionWeightData] | Object[Protocol, PeptideSynthesis][PrimaryWasteWeightData] | Object[Protocol, PeptideSynthesis][SecondaryWasteWeightData] | Object[Protocol, PeptideSynthesis][PurgePressureData] | Object[Protocol, PeptideSynthesis][PurgePressureLog] | Object[Protocol, PeptideSynthesis][InitialPurgePressure] | Object[Protocol, Centrifuge][ContainerWeights] | Object[Protocol, Centrifuge][TareWeight] | Object[Protocol, Centrifuge][CollectionContainerWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerTareWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerWeights] | Object[Protocol, IncubateOld][CurrentTemperature] | Object[Protocol, Incubate][ThawActualTemperature] | Object[Protocol, Incubate][SonicationTemperatureData] | Object[Protocol, IRSpectroscopy][BlankData] | Object[Protocol, LCMS][StandardData] | Object[Protocol, LCMS][BlankData] | Object[Protocol, LCMS][PrimeData] | Object[Protocol, LCMS][FlushData] | Object[Protocol, LCMS][SystemPrimeData] | Object[Protocol, LCMS][SystemFlushData] | Object[Protocol, LCMS][CalibrationData] | Object[Protocol, MicrowaveDigestion][ReactionTemperature] | Object[Protocol, MicrowaveDigestion][ReactionPressure] | Object[Protocol, VacuumEvaporation][BucketWeights] | Object[Protocol, VacuumEvaporation][WasteWeightData] | Object[Protocol, SolidPhaseExtraction][PurgePressureLog] | Object[Protocol, SolidPhaseExtraction][InitialPurgePressure] | Object[Protocol, SolidPhaseExtraction][WasteWeightData] | Object[Protocol, SupercriticalFluidChromatography][StandardData] | Object[Protocol, SupercriticalFluidChromatography][BlankData] | Object[Protocol, SupercriticalFluidChromatography][PrimeData] | Object[Protocol, SupercriticalFluidChromatography][FlushData] | Object[Protocol, SupercriticalFluidChromatography][SystemPrimeData] | Object[Protocol, SupercriticalFluidChromatography][SystemFlushData] | Object[Protocol, SupercriticalFluidChromatography][WasteWeightData] | Object[Protocol, Transfer][TareWeights] | Object[Protocol][NitrogenPressureLog] | Object[Protocol][InitialNitrogenPressure] | Object[Protocol][CO2PressureLog] | Object[Protocol][InitialCO2Pressure] | Object[Protocol][ArgonPressureLog] | Object[Protocol][InitialArgonPressure] | Object[Protocol][WasteWeightTare] | Object[Protocol][SecondaryWasteWeightTare] | Object[Protocol][WasteWeight] | Object[Protocol][SecondaryWasteWeight] | Object[Protocol, UVMelting][InitialPurgePressure] | Object[Protocol, UVMelting][PurgePressureLog] | Object[Protocol, UVMelting][AbsorbanceData] | Object[Protocol, Filter][WasteWeightData] | Object[Protocol, MeasureDensity][TareWeights] | Object[Protocol, MeasureDensity][SampleWeights] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, MeasureWeight][ScoutData] | Object[Protocol, MeasureWeight][TareData] | Object[Protocol, MeasureWeight][ResidueWeightData] | Object[Protocol, MeasureWeight][TareWeights] | Object[Protocol, qPCR][StandardData] | Object[Protocol, qPCR][BlankData] | Object[Protocol, CapillaryELISA][StandardData] | Object[Protocol, ELISA][StandardData] | Object[Protocol, ELISA][BlankData] | Object[Protocol, ELISA][PrereadData] | Object[Protocol, RNASynthesis][DeblockSolutionWeightData] | Object[Protocol, RNASynthesis][WashSolutionWeightData] | Object[Protocol, RNASynthesis][InitialPurgePressure] | Object[Protocol, MeasureOsmolality][ControlData] | Object[Protocol, MeasureOsmolality][VoidControlData] | Object[Protocol, MeasureOsmolality][PostRunInstrumentContaminationData] | Object[Protocol, Degas][HeliumPressureLog] | Object[Protocol, Degas][ChannelAGasPressure] | Object[Protocol, Degas][ChannelBGasPressure] | Object[Protocol, Degas][ChannelCGasPressure] | Object[Protocol, Degas][ChannelDGasPressure] | Object[Protocol, Degas][VacuumSensorPressure] | Object[Protocol, CapillaryGelElectrophoresisSDS][InjectionTable, Data] | Object[Protocol, CapillaryIsoelectricFocusing][InjectionTable, Data] | Object[Protocol, Nephelometry][BlankMeasurement] | Object[Protocol, NephelometryKinetics][BlankMeasurement] | Object[Protocol, MeasureDensity][AirCheckData] | Object[Protocol, MeasureDensity][WaterCheckData] | Object[Protocol, MeasureRefractiveIndex][Data]
Qualifications
The Qualifications that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification][Data] | Object[Qualification][WasteWeightTare] | Object[Qualification][SecondaryWasteWeightTare] | Object[Qualification][WasteWeight] | Object[Qualification][SecondaryWasteWeight] | Object[Qualification][EnvironmentalData] | Object[Qualification, CalibrateDNASynthesizer][InitialPurgePressure] | Object[Qualification, CalibrateDNASynthesizer][CalibrationWeightData] | Object[Qualification, EngineBenchmark][SelectedSensorData] | Object[Qualification, EngineBenchmark][RecordedSensorData] | Object[Qualification, ELISA][AbsorbanceQualificationData]
Maintenance
The maintenance that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Maintenance][Data] | Object[Maintenance, CalibrateVolume][EmptyDistances] | Object[Maintenance, CalibrateVolume][TareDistance] | Object[Maintenance, CalibratePathLength][EmptyDistances] | Object[Maintenance, CalibratePathLength][StandardDistances] | Object[Maintenance, CalibratePathLength][EmptyAbsorbance] | Object[Maintenance][InitialNitrogenPressure] | Object[Maintenance][NitrogenPressureLog] | Object[Maintenance][InitialCO2Pressure] | Object[Maintenance][CO2PressureLog] | Object[Maintenance][InitialArgonPressure] | Object[Maintenance][ArgonPressureLog] | Object[Maintenance][EnvironmentalData] | Object[Maintenance][WasteWeightTare] | Object[Maintenance][SecondaryWasteWeightTare] | Object[Maintenance][WasteWeight] | Object[Maintenance][SecondaryWasteWeight] | Object[Maintenance, Flush][SystemFlushData]
Instrument
Instrument this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Sensor
Sensor this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sensor][Data]
DataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
AliquotSamples
Aliquot samples that were directly assayed to generate this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
SamplesIn
Samples that were assayed to generate this data. Please note that if the samples were aliquoted, this field will contain the input samples.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SamplesOut
Samples produced in the course of generating this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
Method
The type of experiment nephelometric measurements are collected for. CellCount involves measuring the amount of scattered light from cells suspended in solution in order to quantify the number of cells in solution. CellCountParameterizaton involves measuring the amount of scattered light from a series of diluted samples from a cell culture, and quantifying the cells by another method, such as making titers, plating, incubating, and counting colonies, or by counting with a microscope. A standard curve is then created with the data to relate cell count to nephelometry readings for future cell count quantification. For the method Solubility, scattered light from suspended particles in solution will be measured, at a single time point or over a time period. Reagents can be injected into the samples to study their effects on solubility, and dilutions can be performed to determine the point of saturation.
Format: Single
Class: Expression
Programmatic Pattern: NephelometryMethodTypeP
NominalTemperature
The target temperature on the instrument set by the protocol.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
NominalOxygenLevel
The target oxygen level in the atmosphere inside the instrument set by the protocol.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: GreaterP[0 Percent]
NominalCarbonDioxideLevel
The target carbon dioxide level in the atmosphere inside the instrument set by the protocol.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: GreaterP[0 Percent]
Wavelength
The wavelength of the light source that the sample was illuminated at when data was acquired.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter]
BeamAperture
The diameter of the opening that allows the source laser light to pass through to the sample when the reading was taken.
Format: Single
Class: Real
Unit: 1 millimeter
Programmatic Pattern: GreaterEqualP[0 Millimeter]
BeamIntensity
The percentage of the total amount of the laser source light passed through to hit the sample during the measurement.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: GreaterEqualP[0 Percent]
SettlingTime
The delay time after the instrument moved to a well position before it began taking readings.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
CycleTimes
The time intervals at which readings were taken during the assay.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
KineticWindowDurations
The duration of the time intervals at which readings were taken during the assay.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
PlateModel
The model of the plate containing the sample for which this data was recorded.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Container, Plate]
Wells
For each member of Dilutions, the well number from which the measurement(s) were recorded.
Format: Multiple
Class: String
Programmatic Pattern: WellP
Diluent
The sample that the material being analyzed was diluted in.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
Dilutions
The collection of dilutions performed on the sample before measuring scattered light. This is the volume of the sample and the volume of the diluent that were mixed together for each concentration in the dilution series.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}
InjectionSamples
The samples that were injected into the well.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
InjectionTimes
For each member of InjectionSamples, the time at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
InjectionVolumes
For each member of InjectionSamples, the volume of the samples injected into the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter Micro]
InjectionFlowRates
For each member of InjectionSamples, the flow rate at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 microliter per second
Programmatic Pattern: 0 (Liter Micro)
Hold[GreaterEqualP[---------------]]
Second
Sample Loading
AssayPositions
For each member of the dilution curve, the well position of the sample in the measurement plate.
Format: Multiple
Class: String
Programmatic Pattern: WellPositionP
Experimental Results
TurbidityTrajectories
For each member of Dilutions, the measured turbidities over time in the form: {time, RNU}, with the blank or empty measurements subtracted (if available).
Format: Multiple
Class: QuantityArray
Unit: ['1 second', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Second, ArbitraryUnit}]
Temperatures
For each member of Dilutions, the temperatures in the instrument's sample compartment for the trajectory recorded.
Format: Multiple
Class: QuantityArray
Unit: ['1 second', '1 degree Celsius']
Programmatic Pattern: QuantityCoordinatesP[{Second, Celsius}]
InputDilutedConcentrations
For each member of Dilutions, the concentration of the analyte in the sample.
Format: Multiple
Class: Real
Unit: 1 micromolar
Programmatic Pattern: ConcentrationP
DilutionFactors
For each member of Dilutions, the dilution factors of the undiluted sample and diluted samples that were measured.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
ImageCellsData
The image data captured for the samples if Method->CellCountParameterization.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data, ImageCells]
Data Processing
DataType
Whether this data represents an empty plate, blank, or analyte nephelometry readings. Empty is a measurement of the scattered light from the container with nothing in the well. Blank is a measurement of the scattered light from the container and the blank sample, usually the main solvent or media without analyte. Analyte is a measurement of the scattered light from a sample of interest, from which calculations and analysis about the sample can be made.
Format: Single
Class: Expression
Programmatic Pattern: Empty | Blank | Analyte
UnblankedTurbidity Trajectories
For each member of Dilutions, the raw, unblanked turbidity measurements over time without the blank or empty turbidity measurements subtracted.
Format: Multiple
Class: QuantityArray
Unit: ['1 second', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Second, ArbitraryUnit}]
BlankTurbidityTrajectory
The turbidity measurements over time for a well containing a blank sample used to subtract the background signal from this data.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Second, ArbitraryUnit}]
Blank
The solution used to make a blank measurement without sample in the well used to subtract the background signal from this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
BlankTrajectory
The data for the blank sample used to subtract the background signal from this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data, NephelometryKinetics]
Analysis & Reports
Analyte
The substance whose solubility or cell count over time is measured in this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Molecule] | Model[Molecule, cDNA] | Model[Molecule, Oligomer] | Model[Molecule, Transcript] | Model[Molecule, Protein] | Model[Molecule, Protein, Antibody] | Model[Molecule, Carbohydrate] | Model[Molecule, Polymer] | Model[Resin] | Model[Resin, SolidPhaseSupport] | Model[Lysate] | Model[ProprietaryFormulation] | Model[Virus] | Model[Cell] | Model[Cell, Mammalian] | Model[Cell, Bacteria] | Model[Cell, Yeast] | Model[Tissue] | Model[Material] | Model[Species]
InputConcentration
The concentration of the analyte in the original source sample. This value is separate from any concentration calculations using this nephelometry data.
Format: Single
Class: Real
Unit: 1 micromolar
Programmatic Pattern: ConcentrationP
InputMassConcentration
The calculated mass of the analyte divided by the volume of the mixture in the original source sample. This value is separate from any calculations of concentration done from the nephelometry data.
Format: Single
Class: Real
Unit: 1 microgram per milliliter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
InputDilutedMass Concentrations
For each member of Dilutions, the calculated mass of the analyte divided by the volume of the mixture. These values are separate from any calculations of concentration done from the nephelometry data.
Format: Multiple
Class: Real
Unit: 1 microgram per milliliter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
GrowthCurves
For each member of Dilutions, the number of cells in the sample in the read plate over the time course of the experiment.
Format: Multiple
Class: QuantityArray
Unit: ['1 second', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Second, ArbitraryUnit}]
GrowthCurveAnalyses
Analyses used to calculate cell count trajectory from the turbidity trajectory.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Alternatives[Object[Analysis][Reference]]
SolubilityAnalyses
Analyses used to calculate solubility trajectory of the analyte from the turbidity trajectory.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Alternatives[Object[Analysis][Reference]]
Last modified on Mon 26 Sep 2022 15:43:44