Object[Data, AbsorbanceSpectroscopy]
Measurements of absorbance spectra for a range of wavelengths generated by the plate readers or UV/Vis Spectrophotometers.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, AbsorbanceSpectroscopy, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, AbsorbanceSpectroscopy]
Pattern Description: Object[Data, AbsorbanceSpectroscopy]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Replicates
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Replicates]
General
Protocol
The protocol that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Data] | Object[Protocol][EnvironmentalData] | Object[Protocol, AbsorbanceQuantification][VolumeData] | Object[Protocol, AbsorbanceQuantification][EmptyPlateAbsorbance] | Object[Protocol, AbsorbanceQuantification][BufferAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][BlankAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][InitialPurgePressure] | Object[Protocol, AbsorbanceSpectroscopy][PurgePressureLog] | Object[Protocol, AbsorbanceIntensity][BlankAbsorbance] | Object[Protocol, AbsorbanceIntensity][InitialPurgePressure] | Object[Protocol, AbsorbanceIntensity][PurgePressureLog] | Object[Protocol, AbsorbanceKinetics][BlankAbsorbance] | Object[Protocol, AcousticLiquidHandling][Data] | Object[Protocol, AlphaScreen][GainOptimizationData] | Object[Protocol, AlphaScreen][FocalHeightOptimizationData] | Object[Protocol, BioLayerInterferometry][Data] | Object[Protocol, CyclicVoltammetry][ElectrodePretreatmentData] | Object[Protocol, CyclicVoltammetry][CyclicVoltammetryData] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionData] | Object[Protocol, CyclicVoltammetry][PretreatmentSpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][SpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionSpargingPressureLogs] | Object[Protocol, CircularDichroism][BlankData] | Object[Protocol, CircularDichroism][EmptyData] | Object[Protocol, DNASynthesis][DeblockSolutionWeightData] | Object[Protocol, DNASynthesis][WashSolutionWeightData] | Object[Protocol, DNASynthesis][InitialPurgePressure] | Object[Protocol, DynamicFoamAnalysis][Data] | Object[Protocol, Evaporate][CurrentWasteWeightData] | Object[Protocol, Evaporate][WasteWeightData] | Object[Protocol, Evaporate][BathActualTemperature] | Object[Protocol, Evaporate][BucketWeights] | Object[Protocol, PAGE][LadderData] | Object[Protocol, AgaroseGelElectrophoresis][LadderData] | Object[Protocol, MassSpectrometry][MatrixData] | Object[Protocol, MassSpectrometry][CalibrantData] | Object[Protocol, MassSpectrometry][SystemPrimeData] | Object[Protocol, MassSpectrometry][SystemFlushData] | Object[Protocol, HPLC][StandardData] | Object[Protocol, HPLC][BlankData] | Object[Protocol, HPLC][FlushData] | Object[Protocol, HPLC][PrimeData] | Object[Protocol, HPLC][SystemFlushData] | Object[Protocol, HPLC][SystemPrimeData] | Object[Protocol, HPLC][WasteWeightData] | Object[Protocol, FPLC][StandardData] | Object[Protocol, FPLC][BlankData] | Object[Protocol, FPLC][WasteWeightData] | Object[Protocol, FPLC][PrimeData] | Object[Protocol, FPLC][FlushData] | Object[Protocol, FPLC][SystemPrimeData] | Object[Protocol, FPLC][SystemFlushData] | Object[Protocol, GasChromatography][StandardData] | Object[Protocol, GasChromatography][BlankData] | Object[Protocol, GasChromatography][InitialHeliumPressure] | Object[Protocol, GasChromatography][InitialMethanePressure] | Object[Protocol, GasChromatography][FinalHeliumPressure] | Object[Protocol, GasChromatography][FinalMethanePressure] | Object[Protocol, GasChromatography][InitialHydrogenGeneratorWaterWeight] | Object[Protocol, GasChromatography][FinalHydrogenGeneratorWaterWeight] | Object[Protocol, IonChromatography][StandardData] | Object[Protocol, IonChromatography][AnionStandardData] | Object[Protocol, IonChromatography][CationStandardData] | Object[Protocol, IonChromatography][BlankData] | Object[Protocol, IonChromatography][AnionBlankData] | Object[Protocol, IonChromatography][CationBlankData] | Object[Protocol, IonChromatography][FlushData] | Object[Protocol, IonChromatography][AnionFlushData] | Object[Protocol, IonChromatography][CationFlushData] | Object[Protocol, IonChromatography][PrimeData] | Object[Protocol, IonChromatography][AnionPrimeData] | Object[Protocol, IonChromatography][CationPrimeData] | Object[Protocol, IonChromatography][SystemFlushData] | Object[Protocol, IonChromatography][AnionSystemFlushData] | Object[Protocol, IonChromatography][CationSystemFlushData] | Object[Protocol, IonChromatography][SystemPrimeData] | Object[Protocol, IonChromatography][AnionSystemPrimeData] | Object[Protocol, IonChromatography][CationSystemPrimeData] | Object[Protocol, IonChromatography][WasteWeightData] | Object[Protocol, ImageSample][RulerImageData] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, PNASynthesis][WashSolutionWeightData] | Object[Protocol, PNASynthesis][PrimaryWasteWeightData] | Object[Protocol, PNASynthesis][SecondaryWasteWeightData] | Object[Protocol, PNASynthesis][PurgePressureData] | Object[Protocol, PNASynthesis][PurgePressureLog] | Object[Protocol, PNASynthesis][InitialPurgePressure] | Object[Protocol, PeptideSynthesis][WashSolutionWeightData] | Object[Protocol, PeptideSynthesis][PrimaryWasteWeightData] | Object[Protocol, PeptideSynthesis][SecondaryWasteWeightData] | Object[Protocol, PeptideSynthesis][PurgePressureData] | Object[Protocol, PeptideSynthesis][PurgePressureLog] | Object[Protocol, PeptideSynthesis][InitialPurgePressure] | Object[Protocol, Centrifuge][ContainerWeights] | Object[Protocol, Centrifuge][TareWeight] | Object[Protocol, Centrifuge][CollectionContainerWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerTareWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerWeights] | Object[Protocol, IncubateOld][CurrentTemperature] | Object[Protocol, Incubate][ThawActualTemperature] | Object[Protocol, Incubate][SonicationTemperatureData] | Object[Protocol, IRSpectroscopy][BlankData] | Object[Protocol, LCMS][StandardData] | Object[Protocol, LCMS][BlankData] | Object[Protocol, LCMS][PrimeData] | Object[Protocol, LCMS][FlushData] | Object[Protocol, LCMS][SystemPrimeData] | Object[Protocol, LCMS][SystemFlushData] | Object[Protocol, LCMS][CalibrationData] | Object[Protocol, MicrowaveDigestion][ReactionTemperature] | Object[Protocol, MicrowaveDigestion][ReactionPressure] | Object[Protocol, VacuumEvaporation][BucketWeights] | Object[Protocol, VacuumEvaporation][WasteWeightData] | Object[Protocol, SolidPhaseExtraction][PurgePressureLog] | Object[Protocol, SolidPhaseExtraction][InitialPurgePressure] | Object[Protocol, SolidPhaseExtraction][WasteWeightData] | Object[Protocol, SupercriticalFluidChromatography][StandardData] | Object[Protocol, SupercriticalFluidChromatography][BlankData] | Object[Protocol, SupercriticalFluidChromatography][PrimeData] | Object[Protocol, SupercriticalFluidChromatography][FlushData] | Object[Protocol, SupercriticalFluidChromatography][SystemPrimeData] | Object[Protocol, SupercriticalFluidChromatography][SystemFlushData] | Object[Protocol, SupercriticalFluidChromatography][WasteWeightData] | Object[Protocol, Transfer][TareWeights] | Object[Protocol][NitrogenPressureLog] | Object[Protocol][InitialNitrogenPressure] | Object[Protocol][CO2PressureLog] | Object[Protocol][InitialCO2Pressure] | Object[Protocol][ArgonPressureLog] | Object[Protocol][InitialArgonPressure] | Object[Protocol][WasteWeightTare] | Object[Protocol][SecondaryWasteWeightTare] | Object[Protocol][WasteWeight] | Object[Protocol][SecondaryWasteWeight] | Object[Protocol, UVMelting][InitialPurgePressure] | Object[Protocol, UVMelting][PurgePressureLog] | Object[Protocol, UVMelting][AbsorbanceData] | Object[Protocol, Filter][WasteWeightData] | Object[Protocol, MeasureDensity][TareWeights] | Object[Protocol, MeasureDensity][SampleWeights] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, MeasureWeight][ScoutData] | Object[Protocol, MeasureWeight][TareData] | Object[Protocol, MeasureWeight][ResidueWeightData] | Object[Protocol, MeasureWeight][TareWeights] | Object[Protocol, qPCR][StandardData] | Object[Protocol, qPCR][BlankData] | Object[Protocol, CapillaryELISA][StandardData] | Object[Protocol, ELISA][StandardData] | Object[Protocol, ELISA][BlankData] | Object[Protocol, ELISA][PrereadData] | Object[Protocol, RNASynthesis][DeblockSolutionWeightData] | Object[Protocol, RNASynthesis][WashSolutionWeightData] | Object[Protocol, RNASynthesis][InitialPurgePressure] | Object[Protocol, MeasureOsmolality][ControlData] | Object[Protocol, MeasureOsmolality][VoidControlData] | Object[Protocol, MeasureOsmolality][PostRunInstrumentContaminationData] | Object[Protocol, Degas][HeliumPressureLog] | Object[Protocol, Degas][ChannelAGasPressure] | Object[Protocol, Degas][ChannelBGasPressure] | Object[Protocol, Degas][ChannelCGasPressure] | Object[Protocol, Degas][ChannelDGasPressure] | Object[Protocol, Degas][VacuumSensorPressure] | Object[Protocol, CapillaryGelElectrophoresisSDS][InjectionTable, Data] | Object[Protocol, CapillaryIsoelectricFocusing][InjectionTable, Data] | Object[Protocol, Nephelometry][BlankMeasurement] | Object[Protocol, NephelometryKinetics][BlankMeasurement] | Object[Protocol, MeasureDensity][AirCheckData] | Object[Protocol, MeasureDensity][WaterCheckData] | Object[Protocol, MeasureRefractiveIndex][Data]
Qualifications
The Qualifications that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification][Data] | Object[Qualification][WasteWeightTare] | Object[Qualification][SecondaryWasteWeightTare] | Object[Qualification][WasteWeight] | Object[Qualification][SecondaryWasteWeight] | Object[Qualification][EnvironmentalData] | Object[Qualification, CalibrateDNASynthesizer][InitialPurgePressure] | Object[Qualification, CalibrateDNASynthesizer][CalibrationWeightData] | Object[Qualification, EngineBenchmark][SelectedSensorData] | Object[Qualification, EngineBenchmark][RecordedSensorData] | Object[Qualification, ELISA][AbsorbanceQualificationData]
Maintenance
The maintenance that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Maintenance][Data] | Object[Maintenance, CalibrateVolume][EmptyDistances] | Object[Maintenance, CalibrateVolume][TareDistance] | Object[Maintenance, CalibratePathLength][EmptyDistances] | Object[Maintenance, CalibratePathLength][StandardDistances] | Object[Maintenance, CalibratePathLength][EmptyAbsorbance] | Object[Maintenance][InitialNitrogenPressure] | Object[Maintenance][NitrogenPressureLog] | Object[Maintenance][InitialCO2Pressure] | Object[Maintenance][CO2PressureLog] | Object[Maintenance][InitialArgonPressure] | Object[Maintenance][ArgonPressureLog] | Object[Maintenance][EnvironmentalData] | Object[Maintenance][WasteWeightTare] | Object[Maintenance][SecondaryWasteWeightTare] | Object[Maintenance][WasteWeight] | Object[Maintenance][SecondaryWasteWeight] | Object[Maintenance, Flush][SystemFlushData]
Instrument
Instrument this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Sensor
Sensor this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sensor][Data]
DataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
AliquotSamples
Aliquot samples that were directly assayed to generate this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
SamplesIn
Samples that were assayed to generate this data. Please note that if the samples were aliquoted, this field will contain the input samples.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SamplesOut
Samples produced in the course of generating this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
NominalTemperature
Indicates the target temperature the samples are held at prior to and during data acquisition within the instrument.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
PositioningDelay
The delay time after the instrument moved to a well position before it began taking readings.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
NumberOfReadings
The number of readings taken and averaged to generate the spectrum for the sample.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
PlateModel
The model of the plate/microfluidic chip/cuvette containing the samples used in the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
CuvetteModel
The model of the cuvette containing the sample(s) and through which the light beam is passed during the absorbance measurement.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Container, Cuvette]
CuvetteNumber
The position of the sample's cuvette inside the heatblock of the spectrophotometer instrument during absorbance measurement.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
SampleVolume
For each member of SamplesIn, the amount of liquid of each sample within its container used in this assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Microliter]
BufferModel
The buffer used in the assay.
Format: Single
Class: Link
Programmatic Pattern: _Link
Well
The well number from which the spectrum was recorded.
Format: Single
Class: String
Programmatic Pattern: WellP
InjectionSamples
The samples that were injected into the well.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
InjectionTimes
For each member of InjectionSamples, the time at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
InjectionVolumes
For each member of InjectionSamples, the volume of the samples injected into the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter Micro]
InjectionFlowRates
For each member of InjectionSamples, the flow rate at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 microliter per second
Programmatic Pattern: 0 (Liter Micro)
Hold[GreaterEqualP[---------------]]
Second
RawAbsorbanceDataFile
The file containing the raw unprocessed data generated by the instrument without any smoothing.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Absorbance Measurement
SpectralBandwidth
When using the Cuvette Method, indicates the physical size of the slit from which light passes out from the monochromator. The narrower the bandwidth, the greater the resolution in measurements.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: RangeP[0.5 Nanometer, 5 Nanometer]
MinWavelength
The minimum wavelength at which absorbance data was acquired.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter]
MaxWavelength
The maximum wavelength at which absorbance data was acquired.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter]
AcquisitionMixRate
Indicates if the rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
NominalAcquisitionMixRate
Indicates the target/set rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
ActualAcquisitionMixRate
Indicates the absolute/exact rate at which the samples within the cuvette attained during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
StirringError
Indicates if stable stirring was not achieved within the cuvette after MaxStirAttempts during the setup of the assay.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Experimental Results
AbsorbanceSpectrum
The absorbance spectrum in the form: {wavelength, absorbance}, with the blank spectra subtracted (if available).
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]
Transmittance
The percent Transmittance in the form: {wavelength, %}, calculated from the absorbance spectrum of the sample after reference blank subtraction (if available).
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 percent']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, Percent}]
Temperature
The temperature in the instrument's sample compartment at each wavelength as it was recorded.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 degree Celsius']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, Celsius}]
EmptySpectrum
The absorbance data from the same well as this data but with nothing in it.
Format: Single
Class: Link
Programmatic Pattern: _Link
BlankSpectrum
A blank absorbance spectra which includes absorbance from the buffer and container but without any analyte present.
Format: Single
Class: Link
Programmatic Pattern: _Link
AnalyteSpectrum
The absorbance data from the same well as this data but with sample in it.
Format: Single
Class: Link
Programmatic Pattern: _Link
AnalyteSpectra
Any absorbance data which use this data as a blank spectra to subtract baseline signal due to the buffer or container rather than the analyte.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Data Processing
RawAbsorbanceSpectrum
The absorbance measured at the wavelength, with the blank absorbance subtracted (if available).
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]
UnblankedAbsorbanceSpectrum
The raw, unblanked absorbance spectrum in the form: {wavelength, unblanked absorbance}.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]
DataType
Whether this data represents an empty plate, blank, or analyte absorbance reading.
Format: Single
Class: Expression
Programmatic Pattern: AbsorbanceSpectroscopyDataTypeP
Analysis & Reports
Analyte
The substance whose concentration is measured in this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Molecule] | Model[Molecule, cDNA] | Model[Molecule, Oligomer] | Model[Molecule, Transcript] | Model[Molecule, Protein] | Model[Molecule, Protein, Antibody] | Model[Molecule, Carbohydrate] | Model[Molecule, Polymer] | Model[Resin] | Model[Resin, SolidPhaseSupport] | Model[Lysate] | Model[ProprietaryFormulation] | Model[Virus] | Model[Cell] | Model[Cell, Mammalian] | Model[Cell, Bacteria] | Model[Cell, Yeast] | Model[Tissue] | Model[Material] | Model[Species]
AbsorbanceSpectrumPeaksSource
A running log of all peak picking conducted on the blanked spectrum.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
Concentration
The concentration of the sample in the source plate, i.e. the original un-diluted sample.
Format: Single
Class: Real
Unit: 1 micromolar
Programmatic Pattern: _?ConcentrationQ
DilutedConcentration
The calculated concentration of the sample in the read plate after the sample has been diluted to be within range of the sensors.
Format: Single
Class: Real
Unit: 1 micromolar
Programmatic Pattern: _?ConcentrationQ
DilutedMassConcentration
The calculated mass concentration of the sample in the read plate after the sample has been diluted to be within range of the sensors.
Format: Single
Class: Real
Unit: 1 gram per liter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
PathLength
The distance that the path of light from the plate reader had to travel to traverse through the sample in the plate.
Format: Single
Class: Real
Unit: 1 millimeter
Programmatic Pattern: _?DistanceQ
PathLengthMethod
The method by which the path length from the plate reader to the sample in the read plate was determined.
Format: Single
Class: Expression
Programmatic Pattern: PathLengthMethodP
SingleCuvetteRead
Indicates that this absorbance data was created using data obtained from only a single microfluidic chip cuvette, when multiple cuvettes should have been read. This may indicate that the data in this read is less accurate than normal.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
QuantificationAnalyses
Quantification of a sample using Beer-Lambert's law that use this absorbance spectrum as source data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SmoothingAnalyses
Smoothing analysis performed on this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
Physical Properties
MassConcentration
The most recently calculated mass of the constituent(s) divided by the volume of the mixture.
Format: Single
Class: Real
Unit: 1 gram per liter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
Last modified on Mon 26 Sep 2022 15:43:44