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Object[Data, CircularDichroism]

Measurements of circular dichroism spectra for a range of wavelengths or several fixed wavelength values generated by the circular dichroism plate readers.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, CircularDichroism, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, CircularDichroism]
    Pattern Description: Object[Data, CircularDichroism]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Data Processing

    DataType

    Whether this data represents an empty plate, blank, or analyte circular dichroism absorbance reading.
    Format: Single
    Class: Expression
    Programmatic Pattern: CircularDichroismDataTypeP

    UnblankedCircularDichroism AbsorbanceSpectrum

    The unblanked circular dichroism spectrum recorded by ellipticity (Millidegree) at every wavelength, based on absorbance differences of the right-handed and left-handed circular polarized light.
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 milli degree']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AngularDegree Milli}]

Analysis & Reports

    AnalyteConcentration

    The known concentration of the analyte in the sample that is recorded.
    Format: Single
    Class: Real
    Unit: 1 micromolar
    Programmatic Pattern: GreaterP[0 Micromolar]

    PathLength

    The distance that the path of light from the plate reader had to travel to traverse through the sample in the plate.
    Format: Single
    Class: Real
    Unit: 1 millimeter
    Programmatic Pattern: GreaterP[0 Centimeter]

    PathLengthMethod

    The method by which the path length from the plate reader to the sample in the read plate was determined.
    Format: Single
    Class: Expression
    Programmatic Pattern: PathLengthMethodP

    CircularDichroismPeaks Analysis

    Peak picking analyses performed on the CircularDichroismAbsorbanceSpectrum data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    SmoothingAnalyses

    Smoothing analysis performed on this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    StructureQuantification Analyses

    Quantification of the composition of a protein's secondary strucutre that use this circular dichroism spectrum as source data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Object[Analysis][Reference]]

Method Information

    AverageTime

    The time for which data was collected for each reading. The collected data are averaged for this period of time to obtain the CircularDichroismAbsorbanceSpectrum.
    Format: Single
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterEqualP[0 Second]

Experimental Results

    AbsorbanceSpectrum

    The spectrum records absorbance at each wavelength, with the blank spectra subtracted (if available).
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]

    AbsorbanceDifferenceSpectrum

    The spectrum records absorbance differences of the right-handed and left-handed circular polarized light at each wavelength, with the blank spectra subtracted (if available).
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]

    CircularDichroismAbsorbance Spectrum

    The spectrum records circular dichroism value (ellipiticity) recorded by measuring the absorbance differences of the right-handed and left-handed circular polarized light at each wavelength, with the blank spectra subtracted (if available).
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 milli degree']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AngularDegree Milli}]

    CircularDichroismSpectrum

    The spectrum records molar ellipiticity (corrected circular dichroism value) for every wavelength. The molar ellipticity is calculated from divide the ellipticity value by the analyte's molar concentration and path length of the light.
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 centimeter squared degree per deci millimole']
    Programmatic Pattern: 2 Centimeter Hold[QuantityCoordinatesP[{Nanometer, AngularDegree -----------}]] Mole Deci

    UnblankedAbsorbanceSpectrum

    The spectrum records absorbance at each wavelength, with the blank spectra subtracted (if available).
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]

    UnblankedAbsorbanceDifference Spectrum

    The spectrum records absorbance differences of the right-handed and left-handed circular polarized light at each wavelength, with the blank spectra subtracted (if available).
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]

    EmptySpectrum

    The circular dichroism absorbance data from the same well as this data but with nothing in it.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, CircularDichroism][BlankSpectrum] | Object[Data, CircularDichroism][AnalyteSpectra]

    BlankSpectrum

    A blank circular dichroism absorbance spectra which includes circular dichroism absorbance from the buffer and container but without any analyte present.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, CircularDichroism][EmptySpectrum] | Object[Data, CircularDichroism][AnalyteSpectra]

    AnalyteSpectra

    Any circular dichroism absorbance data which use this data as a blank spectra to subtract baseline signal due to the buffer or container rather than the analyte.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, CircularDichroism][BlankSpectrum] | Object[Data, CircularDichroism][EmptySpectrum]

    MethodFile

    The file containing parameters dictating how the instrument should perform the qPCR run.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

Enantiomeric Excess Analysis

    EnantiomericExcessWavelengths

    The wavelength will be used to determined enantiomeric excess of SamplesIn.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    EnantiomericExcessStandards

    Input samples with a known enantiomeric excess values and used as standards for analyze the enantiomeric excess of the sample.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample] | Model[Sample]

    EnantiomericExcessStandard Spectrum

    For each member of EnantiomericExcessStandards, the full spectrum (if any) of the collected circular dichroism data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Object[Data, CircularDichroism]]

    EnantiomericExcessStandard Table

    For each member of EnantiomericExcessStandards, the table of information for the standards used in the EnantiomericExcess measurments.
    Format: Multiple

    [[1]] Wavelengths

      Header: Wavelengths
      Class: Real
      Unit: 1 nanometer
      Programmatic Pattern: GreaterP[0 Nanometer]

    [[2]] Enantiomeric Excess Value

      Header: Enantiomeric Excess Value
      Class: Real
      Unit: 1 percent
      Programmatic Pattern: RangeP[-100 Percent, 100 Percent]

    [[3]] Circular Dichroism Value

      Header: Circular Dichroism Value
      Class: Real
      Unit: 1 milli degree
      Programmatic Pattern: UnitsP[0 AngularDegree Milli]

    EnantiomericExcessDataType

    The type of the data for quantifying enantiomeric excess value: a standard sample with a known enantiomeric excess value or a analyte sample that its enantiomeric excess needs to be quantified.
    Format: Single
    Class: Expression
    Programmatic Pattern: EnantiomericExcessDataTypeP

    EnantiomericExcessIntensities

    The intensities at EnantiomericExcessWavelengths that are used to calculate EnantiomericExcessResult of this sample.
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 milli degree']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AngularDegree Milli}]

    EnantiomericExcessResults

    The calculated enatiomeric excess result from linear fit of EnantiomericExcessStandardIntensities with different EnantiomericExcessStandardValues.
    Format: Multiple
    Class: Real
    Programmatic Pattern: UnitsP[Percent]
Last modified on Mon 26 Sep 2022 15:43:44
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