Object[Data, AbsorbanceIntensity]
Measurements of absorbance intensity for a wavelength generated by the plate readers or UV/Vis Spectrophotometers.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, AbsorbanceIntensity, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, AbsorbanceIntensity]
Pattern Description: Object[Data, AbsorbanceIntensity]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Replicates
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Replicates]
General
Protocol
The protocol that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Data] | Object[Protocol][EnvironmentalData] | Object[Protocol, AbsorbanceQuantification][VolumeData] | Object[Protocol, AbsorbanceQuantification][EmptyPlateAbsorbance] | Object[Protocol, AbsorbanceQuantification][BufferAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][BlankAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][InitialPurgePressure] | Object[Protocol, AbsorbanceSpectroscopy][PurgePressureLog] | Object[Protocol, AbsorbanceIntensity][BlankAbsorbance] | Object[Protocol, AbsorbanceIntensity][InitialPurgePressure] | Object[Protocol, AbsorbanceIntensity][PurgePressureLog] | Object[Protocol, AbsorbanceKinetics][BlankAbsorbance] | Object[Protocol, AcousticLiquidHandling][Data] | Object[Protocol, AlphaScreen][GainOptimizationData] | Object[Protocol, AlphaScreen][FocalHeightOptimizationData] | Object[Protocol, BioLayerInterferometry][Data] | Object[Protocol, CyclicVoltammetry][ElectrodePretreatmentData] | Object[Protocol, CyclicVoltammetry][CyclicVoltammetryData] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionData] | Object[Protocol, CyclicVoltammetry][PretreatmentSpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][SpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionSpargingPressureLogs] | Object[Protocol, CircularDichroism][BlankData] | Object[Protocol, CircularDichroism][EmptyData] | Object[Protocol, DNASynthesis][DeblockSolutionWeightData] | Object[Protocol, DNASynthesis][WashSolutionWeightData] | Object[Protocol, DNASynthesis][InitialPurgePressure] | Object[Protocol, DynamicFoamAnalysis][Data] | Object[Protocol, Evaporate][CurrentWasteWeightData] | Object[Protocol, Evaporate][WasteWeightData] | Object[Protocol, Evaporate][BathActualTemperature] | Object[Protocol, Evaporate][BucketWeights] | Object[Protocol, PAGE][LadderData] | Object[Protocol, AgaroseGelElectrophoresis][LadderData] | Object[Protocol, MassSpectrometry][MatrixData] | Object[Protocol, MassSpectrometry][CalibrantData] | Object[Protocol, MassSpectrometry][SystemPrimeData] | Object[Protocol, MassSpectrometry][SystemFlushData] | Object[Protocol, HPLC][StandardData] | Object[Protocol, HPLC][BlankData] | Object[Protocol, HPLC][FlushData] | Object[Protocol, HPLC][PrimeData] | Object[Protocol, HPLC][SystemFlushData] | Object[Protocol, HPLC][SystemPrimeData] | Object[Protocol, HPLC][WasteWeightData] | Object[Protocol, FPLC][StandardData] | Object[Protocol, FPLC][BlankData] | Object[Protocol, FPLC][WasteWeightData] | Object[Protocol, FPLC][PrimeData] | Object[Protocol, FPLC][FlushData] | Object[Protocol, FPLC][SystemPrimeData] | Object[Protocol, FPLC][SystemFlushData] | Object[Protocol, GasChromatography][StandardData] | Object[Protocol, GasChromatography][BlankData] | Object[Protocol, GasChromatography][InitialHeliumPressure] | Object[Protocol, GasChromatography][InitialMethanePressure] | Object[Protocol, GasChromatography][FinalHeliumPressure] | Object[Protocol, GasChromatography][FinalMethanePressure] | Object[Protocol, GasChromatography][InitialHydrogenGeneratorWaterWeight] | Object[Protocol, GasChromatography][FinalHydrogenGeneratorWaterWeight] | Object[Protocol, IonChromatography][StandardData] | Object[Protocol, IonChromatography][AnionStandardData] | Object[Protocol, IonChromatography][CationStandardData] | Object[Protocol, IonChromatography][BlankData] | Object[Protocol, IonChromatography][AnionBlankData] | Object[Protocol, IonChromatography][CationBlankData] | Object[Protocol, IonChromatography][FlushData] | Object[Protocol, IonChromatography][AnionFlushData] | Object[Protocol, IonChromatography][CationFlushData] | Object[Protocol, IonChromatography][PrimeData] | Object[Protocol, IonChromatography][AnionPrimeData] | Object[Protocol, IonChromatography][CationPrimeData] | Object[Protocol, IonChromatography][SystemFlushData] | Object[Protocol, IonChromatography][AnionSystemFlushData] | Object[Protocol, IonChromatography][CationSystemFlushData] | Object[Protocol, IonChromatography][SystemPrimeData] | Object[Protocol, IonChromatography][AnionSystemPrimeData] | Object[Protocol, IonChromatography][CationSystemPrimeData] | Object[Protocol, IonChromatography][WasteWeightData] | Object[Protocol, ImageSample][RulerImageData] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, PNASynthesis][WashSolutionWeightData] | Object[Protocol, PNASynthesis][PrimaryWasteWeightData] | Object[Protocol, PNASynthesis][SecondaryWasteWeightData] | Object[Protocol, PNASynthesis][PurgePressureData] | Object[Protocol, PNASynthesis][PurgePressureLog] | Object[Protocol, PNASynthesis][InitialPurgePressure] | Object[Protocol, PeptideSynthesis][WashSolutionWeightData] | Object[Protocol, PeptideSynthesis][PrimaryWasteWeightData] | Object[Protocol, PeptideSynthesis][SecondaryWasteWeightData] | Object[Protocol, PeptideSynthesis][PurgePressureData] | Object[Protocol, PeptideSynthesis][PurgePressureLog] | Object[Protocol, PeptideSynthesis][InitialPurgePressure] | Object[Protocol, Centrifuge][ContainerWeights] | Object[Protocol, Centrifuge][TareWeight] | Object[Protocol, Centrifuge][CollectionContainerWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerTareWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerWeights] | Object[Protocol, IncubateOld][CurrentTemperature] | Object[Protocol, Incubate][ThawActualTemperature] | Object[Protocol, Incubate][SonicationTemperatureData] | Object[Protocol, IRSpectroscopy][BlankData] | Object[Protocol, LCMS][StandardData] | Object[Protocol, LCMS][BlankData] | Object[Protocol, LCMS][PrimeData] | Object[Protocol, LCMS][FlushData] | Object[Protocol, LCMS][SystemPrimeData] | Object[Protocol, LCMS][SystemFlushData] | Object[Protocol, LCMS][CalibrationData] | Object[Protocol, MicrowaveDigestion][ReactionTemperature] | Object[Protocol, MicrowaveDigestion][ReactionPressure] | Object[Protocol, VacuumEvaporation][BucketWeights] | Object[Protocol, VacuumEvaporation][WasteWeightData] | Object[Protocol, SolidPhaseExtraction][PurgePressureLog] | Object[Protocol, SolidPhaseExtraction][InitialPurgePressure] | Object[Protocol, SolidPhaseExtraction][WasteWeightData] | Object[Protocol, SupercriticalFluidChromatography][StandardData] | Object[Protocol, SupercriticalFluidChromatography][BlankData] | Object[Protocol, SupercriticalFluidChromatography][PrimeData] | Object[Protocol, SupercriticalFluidChromatography][FlushData] | Object[Protocol, SupercriticalFluidChromatography][SystemPrimeData] | Object[Protocol, SupercriticalFluidChromatography][SystemFlushData] | Object[Protocol, SupercriticalFluidChromatography][WasteWeightData] | Object[Protocol, Transfer][TareWeights] | Object[Protocol][NitrogenPressureLog] | Object[Protocol][InitialNitrogenPressure] | Object[Protocol][CO2PressureLog] | Object[Protocol][InitialCO2Pressure] | Object[Protocol][ArgonPressureLog] | Object[Protocol][InitialArgonPressure] | Object[Protocol][WasteWeightTare] | Object[Protocol][SecondaryWasteWeightTare] | Object[Protocol][WasteWeight] | Object[Protocol][SecondaryWasteWeight] | Object[Protocol, UVMelting][InitialPurgePressure] | Object[Protocol, UVMelting][PurgePressureLog] | Object[Protocol, UVMelting][AbsorbanceData] | Object[Protocol, Filter][WasteWeightData] | Object[Protocol, MeasureDensity][TareWeights] | Object[Protocol, MeasureDensity][SampleWeights] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, MeasureWeight][ScoutData] | Object[Protocol, MeasureWeight][TareData] | Object[Protocol, MeasureWeight][ResidueWeightData] | Object[Protocol, MeasureWeight][TareWeights] | Object[Protocol, qPCR][StandardData] | Object[Protocol, qPCR][BlankData] | Object[Protocol, CapillaryELISA][StandardData] | Object[Protocol, ELISA][StandardData] | Object[Protocol, ELISA][BlankData] | Object[Protocol, ELISA][PrereadData] | Object[Protocol, RNASynthesis][DeblockSolutionWeightData] | Object[Protocol, RNASynthesis][WashSolutionWeightData] | Object[Protocol, RNASynthesis][InitialPurgePressure] | Object[Protocol, MeasureOsmolality][ControlData] | Object[Protocol, MeasureOsmolality][VoidControlData] | Object[Protocol, MeasureOsmolality][PostRunInstrumentContaminationData] | Object[Protocol, Degas][HeliumPressureLog] | Object[Protocol, Degas][ChannelAGasPressure] | Object[Protocol, Degas][ChannelBGasPressure] | Object[Protocol, Degas][ChannelCGasPressure] | Object[Protocol, Degas][ChannelDGasPressure] | Object[Protocol, Degas][VacuumSensorPressure] | Object[Protocol, CapillaryGelElectrophoresisSDS][InjectionTable, Data] | Object[Protocol, CapillaryIsoelectricFocusing][InjectionTable, Data] | Object[Protocol, Nephelometry][BlankMeasurement] | Object[Protocol, NephelometryKinetics][BlankMeasurement] | Object[Protocol, MeasureDensity][AirCheckData] | Object[Protocol, MeasureDensity][WaterCheckData] | Object[Protocol, MeasureRefractiveIndex][Data]
Qualifications
The Qualifications that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification][Data] | Object[Qualification][WasteWeightTare] | Object[Qualification][SecondaryWasteWeightTare] | Object[Qualification][WasteWeight] | Object[Qualification][SecondaryWasteWeight] | Object[Qualification][EnvironmentalData] | Object[Qualification, CalibrateDNASynthesizer][InitialPurgePressure] | Object[Qualification, CalibrateDNASynthesizer][CalibrationWeightData] | Object[Qualification, EngineBenchmark][SelectedSensorData] | Object[Qualification, EngineBenchmark][RecordedSensorData] | Object[Qualification, ELISA][AbsorbanceQualificationData]
Maintenance
The maintenance that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Maintenance][Data] | Object[Maintenance, CalibrateVolume][EmptyDistances] | Object[Maintenance, CalibrateVolume][TareDistance] | Object[Maintenance, CalibratePathLength][EmptyDistances] | Object[Maintenance, CalibratePathLength][StandardDistances] | Object[Maintenance, CalibratePathLength][EmptyAbsorbance] | Object[Maintenance][InitialNitrogenPressure] | Object[Maintenance][NitrogenPressureLog] | Object[Maintenance][InitialCO2Pressure] | Object[Maintenance][CO2PressureLog] | Object[Maintenance][InitialArgonPressure] | Object[Maintenance][ArgonPressureLog] | Object[Maintenance][EnvironmentalData] | Object[Maintenance][WasteWeightTare] | Object[Maintenance][SecondaryWasteWeightTare] | Object[Maintenance][WasteWeight] | Object[Maintenance][SecondaryWasteWeight] | Object[Maintenance, Flush][SystemFlushData]
Instrument
Instrument this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Sensor
Sensor this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sensor][Data]
DataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
AliquotSamples
Aliquot samples that were directly assayed to generate this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
SamplesIn
Samples that were assayed to generate this data. Please note that if the samples were aliquoted, this field will contain the input samples.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SamplesOut
Samples produced in the course of generating this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
NominalTemperature
The target temperature on the instrument set by the protocol.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
Wavelength
The wavelength at which absorbance data was acquired.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter]
PositioningDelay
The delay time after the instrument moved to a well position before it began taking readings.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
NumberOfReadings
The number of readings taken and averaged to generate the spectrum for the sample.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
PlateModel
The model of the plate or microfluidic chip containing the samples used in the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
CuvetteModel
The model of the cuvette containing the sample(s) and through which the light beam is passed during the absorbance measurement.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Container, Cuvette]
CuvetteNumber
The position of the sample's cuvette inside the heatblock of the spectrophotometer instrument during absorbance measurement.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
BufferModel
The model of buffer used in the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Well
The well number from which the spectrum was recorded.
Format: Single
Class: String
Programmatic Pattern: WellP
InjectionSamples
The samples that were injected into the well.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
InjectionTimes
For each member of InjectionSamples, the time at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
InjectionVolumes
For each member of InjectionSamples, the volume of the samples injected into the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter Micro]
InjectionFlowRates
For each member of InjectionSamples, the flow rate at which the sample was added to the well.
Format: Multiple
Class: Real
Unit: 1 microliter per second
Programmatic Pattern: 0 (Liter Micro)
Hold[GreaterEqualP[---------------]]
Second
RawAbsorbanceDataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Absorbance Measurement
AcquisitionMixRate
Indicates if the rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
NominalAcquisitionMixRate
Indicates the target/set rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
ActualAcquisitionMixRate
Indicates the absolute/exact rate at which the samples within the cuvette attained during data acquisition.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
StirringError
Indicates if stable stirring was not achieved within the cuvette after MaxStirAttempts during the setup of the assay.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Experimental Results
Absorbance
The absorbance measured at the wavelength, with the blank absorbance subtracted (if available).
Format: Single
Class: Real
Unit: 1 AbsorbanceUnit
Programmatic Pattern: UnitsP[AbsorbanceUnit]
Temperature
The temperature in the instrument's sample compartment at the specified wavelength as it was recorded.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
BlankAbsorbance
A blank absorbance reading which includes absorbance from the buffer and container but without any analyte present.
Format: Single
Class: Link
Programmatic Pattern: _Link
AnalyteAbsorbances
Any absorbance data which use this data as a blank reading to subtract baseline signal due to the buffer or container rather than the analyte.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Blank
A blank absorbance data which includes absorbance from the buffer and container but without any analyte present.
Format: Single
Class: Link
Programmatic Pattern: _Link
Analytes
Any absorbance data which use this data as a blank spectra to subtract baseline signal due to the buffer or container rather than the analyte.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data, AbsorbanceIntensity][Blank]
Data Processing
UnblankedAbsorbance
The raw, unblanked absorbance measured at the wavelength without the blank absorbance subtracted.
Format: Single
Class: Real
Unit: 1 AbsorbanceUnit
Programmatic Pattern: UnitsP[AbsorbanceUnit]
RawAbsorbanceSpectrum
The absorbance measured at the wavelength, without any blank subtraction, baseline correction, or smoothing applied by instrument software.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, AbsorbanceUnit}]
DataType
Whether this data represents an empty plate, blank, or analyte absorbance reading.
Format: Single
Class: Expression
Programmatic Pattern: AbsorbanceSpectroscopyDataTypeP
Analysis & Reports
Analyte
The substance whose concentration is measured in this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Molecule] | Model[Molecule, cDNA] | Model[Molecule, Oligomer] | Model[Molecule, Transcript] | Model[Molecule, Protein] | Model[Molecule, Protein, Antibody] | Model[Molecule, Carbohydrate] | Model[Molecule, Polymer] | Model[Resin] | Model[Resin, SolidPhaseSupport] | Model[Lysate] | Model[ProprietaryFormulation] | Model[Virus] | Model[Cell] | Model[Cell, Mammalian] | Model[Cell, Bacteria] | Model[Cell, Yeast] | Model[Tissue] | Model[Material] | Model[Species]
Concentration
The concentration of the sample in the source plate, i.e. the original un-diluted sample.
Format: Single
Class: Real
Unit: 1 micromolar
Programmatic Pattern: _?ConcentrationQ
DilutedConcentration
The calculated concentration of the sample in the read plate after the sample has been diluted to be within range of the sensors.
Format: Single
Class: Real
Unit: 1 micromolar
Programmatic Pattern: _?ConcentrationQ
DilutedMassConcentration
The calculated mass concentration of the sample in the read plate after the sample has been diluted to be within range of the sensors.
Format: Single
Class: Real
Unit: 1 gram per liter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
PathLength
The distance that the path of light from the plate reader had to travel to traverse through the sample in the plate.
Format: Single
Class: Real
Unit: 1 millimeter
Programmatic Pattern: _?DistanceQ
PathLengthMethod
The method by which the path length from the plate reader to the sample in the read plate was determined.
Format: Single
Class: Expression
Programmatic Pattern: PathLengthMethodP
SingleCuvetteRead
Indicates that this absorbance data was created using data obtained from only a single microfluidic chip cuvette, when multiple cuvettes should have been read. This may indicate that the data in this read is less accurate than normal.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
QuantificationAnalyses
Quantification of a sample using Beer-Lambert's law that use this absorbance spectrum as source data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Physical Properties
MassConcentration
The most recently calculated mass of the constituent(s) divided by the volume of the mixture.
Format: Single
Class: Real
Unit: 1 gram per liter
Programmatic Pattern: 0 Gram
Hold[GreaterEqualP[------]]
Liter
Last modified on Mon 26 Sep 2022 15:43:44