ecl-logo Documentation

Object[Analysis, DynamicLightScattering]

Analysis of a dynamic light scattering experiment that calculates properties like average particle sizes, diffusion coefficients, diffusion interaction parameter, second virial coefficient, and Kirkwood Buff integral.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Analysis, DynamicLightScattering, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Analysis, DynamicLightScattering]
    Pattern Description: Object[Analysis, DynamicLightScattering]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Author

    The person who ran the analysis.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[User]

General

Analysis & Reports

    DiffusionCoefficients

    For each member of AssayConditions, the diffusion coefficients for each member of CorrelationCurves in the data object.
    Format: Multiple
    Class: VariableUnit
    Programmatic Pattern: 2 Centimeter Hold[GreaterEqualP[0 -----------]] Second

    ZAverageDiameters

    For each member of AssayConditions, the average particle diameters in solution as measured by their intensity distributions.
    Format: Multiple
    Class: VariableUnit
    Programmatic Pattern: GreaterEqualP[0 Nano Meter]

    PeakSpecificDiameters

    For each member of AssayConditions, the peak specific particle diameters in solution as measured by their intensity distributions.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterEqualP[0 Nano Meter]..}

    PolyDispersityIndices

    For each member of AssayConditions, the polydispersity index for that sample.
    Format: Multiple
    Class: Real
    Programmatic Pattern: GreaterEqualP[0]

    DiffusionInteractionParameter Statistics

    The fit for the interaction parameter (kD) associated with the average particle size. The diffusion interaction parameter (kD) is a measure of colloidal stability at lower protein concentrations (<10 mg/mL). A positive kD value indicates weak net-repulsive intermolecular forces between particles, while a negative kD value indicates net-attractive interactions.
    Format: Single

    [DiffusionInteractionParameter]

      Header: DiffusionInteractionParameter
      Class: Real
      Unit: 1 milliliter per gram

    [Slope]

      Header: Slope
      Class: Real
      Unit: 1 meter squared milliliter per gram second

    [Intercept]

      Header: Intercept
      Class: Real
      Unit: 1 meter squared per second

    [RSquared]

      Header: RSquared
      Class: Real

    [HydrodynamicDiameter]

      Header: HydrodynamicDiameter
      Class: Real
      Unit: 1 nanometer

    DiffusionInteractionParameter Fit

    The object that stores linear fit of the dominant peak particle diffusion data versus concentration. The ratio of the slope to y-intercept describes the diffusion interaction parameter. The y-intercept is the limit of diffusion in low concentration, and that value is used to estimate the hydrodyanmic diameter using the Stokes-Einstein equation.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[Analysis, Fit]]
    Relation: Object[Analysis, Fit]

    DominantPeakDiffusion InteractionParameter Statistics

    The fit for the interaction parameter (kD) associated with the peak specific diffusion rates.
    Format: Multiple

    [[1]] Diffusion Interaction Parameter (kD)

      Header: Diffusion Interaction Parameter (kD)
      Class: Real
      Unit: 1 milliliter per gram

    [[2]] kD Slope

      Header: kD Slope
      Class: Real
      Unit: 1 meter squared milliliter per gram second

    [[3]] kD Intercept

      Header: kD Intercept
      Class: Real
      Unit: 1 meter squared per second

    [[4]] R Squared

      Header: R Squared
      Class: Real

    [[5]] Hydrodynamic diameter

      Header: Hydrodynamic diameter
      Class: Real
      Unit: 1 nanometer

    DominantPeakDiffusion InteractionParameterFit

    The object that stores the linear fit of the dominant peak particle diffusion data versus concentration. The ratio of the slope to y-intercept describes the diffusion interaction parameter. The y-intercept is the limit of diffusion in low concentration, and that value is used to estimate the hydrodyanmic diameter using the Stokes-Einstein equation.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[Analysis, Fit]]
    Relation: Object[Analysis, Fit]

    SecondVirialCoefficient Statistics

    The fit for the second virial coefficient. The second virial coefficient (B22) is a measure of colloidal stability at lower protein concentrations (<10 mg/mL). A positive B22 value indicates weak net-repulsive intermolecular forces between particles, while a negative B22 value inidicates net-attractive interactions.
    Format: Single

    [SecondVirialCoefficient]

      Header: SecondVirialCoefficient
      Class: Real
      Unit: 1 milliliter mole per gram squared

    [Slope]

      Header: Slope
      Class: Real
      Unit: 1 milliliter mole per gram squared

    [Intercept]

      Header: Intercept
      Class: Real
      Unit: 1 mole per gram

    [RSquared]

      Header: RSquared
      Class: Real

    [ApparentMolecularWeight]

      Header: ApparentMolecularWeight
      Class: Real
      Unit: 1 gram per mole

    SecondVirialCoefficientFit

    The object that stores the linear fit of the product of the optical constant, concentration, and inverse Rayleigh ratio versus concentration. The second virial coefficient is estimated as half of the slope. The apparent molecular weight is the inverse of the y-intercept.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[Analysis, Fit]]
    Relation: Object[Analysis, Fit]

    KirkwoodBuffIntegral Statistics

    The fit for the Kirkwood Buff integral. The Kirkwood Buff integral (G22) is a measure of colloidal stability at higher protein concentrations. A negative G22 value indicates weak net-repulsive intermolecular forces between particles, while a positive G22 value inidicates net-attractive interactions.
    Format: Single

    [KirkwoodBuffIntegral]

      Header: KirkwoodBuffIntegral
      Class: Real
      Unit: 1 milliliter per gram

    [Slope]

      Header: Slope
      Class: Real
      Unit: 1 gram per mole

    [Intercept]

      Header: Intercept
      Class: Real
      Unit: 1 milliliter per mole

    [RSquared]

      Header: RSquared
      Class: Real

    [ApparentMolecularWeight]

      Header: ApparentMolecularWeight
      Class: Real
      Unit: 1 gram per mole

    KirkwoodBuffIntegralFit

    The object that stores the fit of the Rayleigh ratio divided by the optical constant versus a first and second order protein concentration term. The Kirkwood Buff integral second order term divided by the protein's molecular weight. The apparent molecular weight is the coefficient for the first order term.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[Analysis, Fit]]
    Relation: Object[Analysis, Fit]

    SizeDistributions

    For each member of AssayConditions, the distribution of particle sizes fit to the correlation curves.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 nanometer', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]

Experimental Results

    AverageViscosity

    The average viscosity for the sample.
    Format: Single
    Class: VariableUnit
    Programmatic Pattern: GreaterEqualP[0 Centipoise]

    RefractiveIndex

    The refractive index of the solvent in the sample.
    Format: Single
    Class: Real
    Programmatic Pattern: _?NumericQ

    CalibrationStandardIntensity

    The amount of light that reaches the scattered light detector in counts per second for the calibration toluene sample.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterEqualP[0]

    CalibrationDate

    The date of the last instrument calibration.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    OpticalConstantValue

    The constant used to estimate the second virial coefficient, which is a function of the wavelength, solvent refractive index, and the derivative of the refractive index with respect to the sample concentration.
    Format: Single
    Class: VariableUnit
    Programmatic Pattern: 2 Mole Hold[GreaterP[0 Centimeter -----]] 2 Gram

    SolventRayleighRatios

    For each member of AssayConditions, the solvent Rayleigh ratio which is based on light scattering intensity through the sample solvent relative to toluene.
    Format: Multiple
    Class: VariableUnit
    Programmatic Pattern: -6 1 Hold[GreaterP[-10 ----------]] Centimeter

    DerivedScatteredLight Intensities

    For each member of AssayConditions, the derived amount of light that reaches the scattered light detector in counts per second.
    Format: Multiple
    Class: Real
    Programmatic Pattern: GreaterP[0]

    BufferDerivedLightIntensity

    The derived amount of light that reaches the scattered light detector in counts per second for the buffer sample.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterP[0]

    CorrelationCurves

    For each member of AssayConditions, a series of data points which describes the correlation between scattered light intensity over time for the sample. This correlation typically decays from 1 to 0 over time.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 microsecond', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Micro Second, ArbitraryUnit}]

    AssayConditions

    The independent variable used to evaluate correlation curve data. For B22kD and G22 analysis the reference is mass concentration, for IsothermalStability it is time, and for ThermalShift it is the step in the process of the measurement, Initial or Final.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milligram Hold[GreaterEqualP[0 ----------] | GreaterEqualP[0 Second] | Initial | Final] Milliliter
Last modified on Mon 26 Sep 2022 15:43:43
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.