Object[Analysis, MicroscopeOverlay]
Analysis for coloring and combining of a set of fluorescent microscope images with a phase contrast image while varying transparency, contrast, and brightness.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Analysis, MicroscopeOverlay, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Analysis, MicroscopeOverlay]
Pattern Description: Object[Analysis, MicroscopeOverlay]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The person who ran the analysis.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User]
General
UnresolvedOptions
The initial options entered into the analysis function that generated this object, along with the default values for any unspecified options.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
ResolvedOptions
The final options used by the analysis function, after automatic options have been determined from the input and unresolved options.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Reference
The objects containing the source data used in this analysis.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification, PipettingLinearity][FittingAnalysis] | Object[Data, AbsorbanceSpectroscopy][AbsorbanceSpectrumPeaksSource] | Object[Data, AbsorbanceSpectroscopy][QuantificationAnalyses] | Object[Data, AbsorbanceSpectroscopy][SmoothingAnalyses] | Object[Data, AbsorbanceIntensity][QuantificationAnalyses] | Object[Data, AgaroseGelElectrophoresis][SampleElectropherogramPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][MarkerElectropherogramPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][PostSelectionPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][SmoothingAnalyses] | Object[Data, Appearance, Colonies][ColonyAnalysis] | Object[Data, MeltingCurve][MeltingAnalyses] | Object[Data, MeltingCurve][FluorescenceSpectraAnalyses] | Object[Data, MeltingCurve][AggregationAnalyses] | Object[Data, MeltingCurve][ThermalShiftAnalyses] | Object[Data, BioLayerInterferometry][QuantitationAnalysis] | Object[Data, BioLayerInterferometry][KineticsAnalysis] | Object[Data, BioLayerInterferometry][BinningAnalysis] | Object[Data, BioLayerInterferometry][DetectionLimitAnalysis] | Object[Data, CapillaryGelElectrophoresisSDS][LadderAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][SmoothingAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][PeaksAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][RelativeMigrationPeaksAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][FitAnalyses] | Object[Data, CapillaryIsoelectricFocusing][SmoothingAnalyses] | Object[Data, CapillaryIsoelectricFocusing][PeaksAnalyses] | Object[Data, CapillaryIsoelectricFocusing][FitAnalyses] | Object[Data, Chromatography][StandardAnalyses] | Object[Data, Chromatography][FractionPickingAnalysis] | Object[Data, Chromatography][ConductancePeaksAnalyses] | Object[Data, Chromatography][AbsorbancePeaksAnalyses] | Object[Data, Chromatography][SecondaryAbsorbancePeaksAnalyses] | Object[Data, Chromatography][Absorbance3DPeaksAnalyses] | Object[Data, Chromatography][FluorescencePeaksAnalyses] | Object[Data, Chromatography][SecondaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][TertiaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][QuaternaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][ScatteringPeaksAnalyses] | Object[Data, Chromatography][FIDResponsePeaksAnalyses] | Object[Data, Chromatography][ChargePeaksAnalyses] | Object[Data, Chromatography][SmoothingAnalyses] | Object[Data, Chromatography][pHPeaksAnalyses] | Object[Data, Chromatography][RefractiveIndexPeaksAnalysis] | Object[Data, Chromatography][CircularDichroismPeaksAnalysis] | Object[Data, Chromatography][MultiAngleLightScatteringAnalyses] | Object[Data, Chromatography][DynamicLightScatteringAnalyses] | Object[Data, ChromatographyMassSpectra][IonAbundance3DPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][IonAbundancePeaksAnalyses] | Object[Data, ChromatographyMassSpectra][MassSpectrumPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][AbsorbancePeaksAnalyses] | Object[Data, ChromatographyMassSpectra][Absorbance3DPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][DownsamplingAnalyses] | Object[Data, ChromatographyMassSpectra][SmoothingAnalyses] | Object[Data, CircularDichroism][SmoothingAnalyses] | Object[Data, CircularDichroism][StructureQuantificationAnalyses] | Object[Data, DifferentialScanningCalorimetry][HeatingCurvePeaksAnalyses] | Object[Data, DifferentialScanningCalorimetry][SmoothingAnalyses] | Object[Data, DNASequencing][SequenceAnalyses] | Object[Data, DynamicFoamAnalysis][BubbleRadiusAnalyses] | Object[Data, FlowCytometry][FlowCytometryAnalyses] | Object[Data, FluorescenceKinetics][RateFittingAnalyses] | Object[Data, FluorescencePolarizationKinetics][RateFittingAnalyses] | Object[Data, LuminescenceKinetics][RateFittingAnalyses] | Object[Data, FluorescenceSpectroscopy][EmissionSpectrumPeaksAnalyses] | Object[Data, FluorescenceSpectroscopy][ExcitationSpectrumPeaksAnalyses] | Object[Data, FluorescenceSpectroscopy][SmoothingAnalyses] | Object[Data, LuminescenceSpectroscopy][PeaksAnalyses] | Object[Data, LuminescenceSpectroscopy][SmoothingAnalyses] | Object[Data, MeltingCurve][InitialIntensityPeaksAnalyses] | Object[Data, MeltingCurve][FinalIntensityPeaksAnalyses] | Object[Data, MeltingCurve][InitialMassPeaksAnalyses] | Object[Data, MeltingCurve][FinalMassPeaksAnalyses] | Object[Data, MeltingCurve][DynamicLightScatteringAnalyses] | Object[Data, FluorescenceThermodynamics][MeltingAnalyses] | Object[Data, MassSpectrometry][MassSpectrumPeaksAnalyses] | Object[Data, MassSpectrometry][SmoothingAnalyses] | Object[Data, Microscope][AdjustmentAnalyses] | Object[Data, Microscope][CellCountAnalyses] | Object[Data, Microscope][MicroscopeOverlay] | Object[Data, ImageCells][CellCountAnalyses] | Object[Data, ImageCells][MicroscopeOverlay] | Object[Data, Nephelometry][QuantificationAnalyses] | Object[Data, NephelometryKinetics][GrowthCurveAnalyses] | Object[Data, NephelometryKinetics][SolubilityAnalyses] | Object[Data, NMR][NMRSpectrumPeaksAnalyses] | Object[Data, NMR][SmoothingAnalyses] | Object[Data, NMR2D][NMRSpectrumPeaksAnalyses] | Object[Data, PAGE][LanePeaksAnalyses] | Object[Data, PAGE][LadderAnalyses] | Object[Data, PAGE][SmoothingAnalyses] | Object[Data, qPCR][QuantificationCycleAnalyses] | Object[Data, TotalProteinDetection][FitSourceSpectra] | Object[Data, TotalProteinDetection][MassSpectrumPeaksAnalyses] | Object[Data, TotalProteinDetection][LadderAnalyses] | Object[Data, TLC][LanePeaksAnalyses] | Object[Data, TLC][SmoothingAnalyses] | Object[Data, Volume][QuantificationAnalyses] | Object[Data, Western][FitSourceSpectra] | Object[Data, Western][MassSpectrumPeaksAnalyses] | Object[Data, Western][LadderAnalyses] | Object[Data, Western][SmoothingAnalyses] | Object[Analysis, MeltingPoint][TopBaseline] | Object[Analysis, MeltingPoint][BottomBaseline] | Object[Analysis, MeltingPoint][Thermodynamics] | Object[Analysis, QuantificationCycle][CopyNumberAnalyses] | Object[Analysis, QuantificationCycle][StandardCurveAnalyses] | Object[Analysis, Composition][StandardCurveFitAnalyses] | Object[Analysis, Ladder][StandardFit] | Object[Analysis, Thermodynamics][Fit] | Object[Analysis, Fit][ParallelLineAnalyses] | Object[Data, XRayDiffraction][StructureAnalyses] | Object[Data, XRayDiffraction][DiffractionPeaksAnalyses] | Object[Data, XRayDiffraction][SmoothingAnalyses] | Object[Data, IRSpectroscopy][AbsorbanceSpectrumPeaksSource] | Object[Data, IRSpectroscopy][SmoothingAnalyses] | Object[Protocol, FlowCytometry][CompensationMatrixAnalyses] | Object[Protocol, TotalProteinQuantification][QuantificationAnalyses] | Object[Data, CircularDichroism][CircularDichroismPeaksAnalysis] | Object[Data, DynamicLightScattering][MassDistributionAnalyses] | Object[Data, DynamicLightScattering][IntensityDistributionAnalyses] | Object[Data, DynamicLightScattering][DynamicLightScatteringAnalyses] | Object[DesignOfExperiment][DesignOfExperimentAnalyses] | Object[Protocol, ThermalShift][DynamicLightScatteringLoadingAnalyses] | Object[Protocol, DynamicLightScattering][DynamicLightScatteringLoadingAnalyses] | Object[Data, DifferentialScanningCalorimetry][Analyses]
ReferenceField
The field in the Reference object containing the source data.
Format: Single
Class: Expression
Programmatic Pattern: FieldP[Output -> Short]
BrightnessRed
The brightness adjustment for the red image. All pixel intensities in the red image are offset by this amount.
Format: Single
Class: Real
Programmatic Pattern: RangeP[-1, 1]
BrightnessGreen
The brightness adjustment for the green image. All pixel intensities in the blue image are offset by this amount.
Format: Single
Class: Real
Programmatic Pattern: RangeP[-1, 1]
BrightnessBlue
The brightness adjustment for the blue image. All pixel intensities in the blue image are offset by this amount.
Format: Single
Class: Real
Programmatic Pattern: RangeP[-1, 1]
ContrastRed
The contrast for the red image, defined as the difference between the maximum and minimum intensity of the image. All pixel intensities in the red image are scaled to obtain this contrast value.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 1]
ContrastGreen
The contrast for the green image, defined as the difference between the maximum and minimum intensity of the image. All pixel intensities in the green image are scaled to obtain this contrast value.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 1]
ContrastBlue
The contrast for the blue image, defined as the difference between the maximum and minimum intensity of the image. All pixel intensities in the blue image are scaled to obtain this contrast value.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 1]
FalseColorRed
The red image will display with this color.
Format: Single
Class: Expression
Programmatic Pattern: _?ColorQ
FalseColorGreen
The green image will display with this color.
Format: Single
Class: Expression
Programmatic Pattern: _?ColorQ
FalseColorBlue
The blue image will display with this color.
Format: Single
Class: Expression
Programmatic Pattern: _?ColorQ
Transparency
The transparency applied to the combined false colored images before they are overlaid with the phase contrast image.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 1]
ChannelsOverlaid
The image channels included in the final overlay image.
Format: Multiple
Class: Expression
Programmatic Pattern: Red | Blue | Green | PhaseContrast
Analysis & Reports
Overlay
The final image obtained by combining the colored and adjusted fluorescent images with the phase contrast image.
Format: Computable
Programmatic Pattern: _Image
Expression: SafeEvaluate[{Field[OverlayFile]}, ImportCloudFile[Field[OverlayFile]]]
OverlayFile
The overlay image using the specified analysis parameters.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[EmeraldCloudFile]]
Relation: Object[EmeraldCloudFile]
Last modified on Mon 26 Sep 2022 15:43:43