Analysis By Subject Matter
Collection of analysis functions organized by the subject mater they may be relevant to.
Chromatography
AnalyzePeaks -- Preforms peak picking analysis of experimental source data connecting the results of the analysis to its source data and experimental protocol information.
PlotChromatography -- Generates a line plot of the detector response as a function of time of the provided chromatography data.
AnalyzeFractionsApp -- Selects a set of fractions or all fractions from the source data.
AnalyzeFractions -- Selects a set of fractions to be carried forward for further analysis from chromatography data where fractions were collected.
PlotFractions -- Generates a line plot of detector response vs time from source chromatography data with the time slices representing the time for which fractions were collected highlighted from the provided fraction analysis object.
AnalyzeLadder -- Fits a standard curve function to relate position of a given band or peak to length of a nucleic acid species.
PlotLadder -- Generates a line plot of the strand length as a function of peak position for standard curve analysis of nucleic acids from the provided ladder analysis object.
AnalyzeComposition -- Computes the concentrations of different analytes in assays based on the chromatogram peaks in a HPLC protocol.
Spectroscopy
AnalyzePeaks -- Preforms peak picking analysis of experimental source data connecting the results of the analysis to its source data and experimental protocol information.
AnalyzeAbsorbanceQuantification -- Users Beer's Law to calculate the concentration of an analyte based on its extinction coefficient and absorbance spectra.
PlotAbsorbanceQuantification -- Generates a plot of overlaid absorbance spectroscopy data from replicates reads recorded as part of the given absorbance quantification protocol.
AnalyzeTotalProteinQuantification -- Calculates total protein calculation based on total protein quantification assay data.
AnalyzeDynamicLightScattering -- Calculates key scientific parameters including Z-average diameter, second virial coefficient, diffusion interaction parameter and kirkwood Buff integral from dynamic light scattering data.
AnalyzeDynamicLightScatteringLoading -- Performs thresholding analysis of correlation curve data on dynamic light scattering data objects of a given protocol to identify the samples that were properly loaded.
PlotDynamicLightScattering -- Generates a list line plot of intensity vs. particle size from dynamic light scattering data.
PCR
AnalyzeCopyNumber -- Calculates the estimated copy number of a given gene sequences in an analyte based on qPCR data or Quantification Cycle analysis.
PlotCopyNumber -- Generates a line plot of the fitted standard curve relating quantification cycle (Cq) to Log10[copy number] from the provided copy number analysis object.
AnalyzeQuantificationCycle -- Calculates the Quantification Cycle (Cq) which is the inflection point in the amplification curve of qPCR data.
PlotQuantificationCycle -- Generates a list plot of fluorescence intensity as a function of replication cycle with the quantification cycle (Cq) interpolated and highlighted from the provided quantification cycle analysis object.
Bioassays
AnalyzeBindingKinetics -- Solves the kinetic association and dissociation rates by fitting to the source biolayer interferometry binding kinetics data.
PlotBindingKinetics -- Generates a plot comparing the prediction of fitted binding kinetics parameters to the source bio-layer interferometry data in the provided binding kinetics analysis object.
AnalyzeBindingQuantitation -- Determines the concentration of the analyte measured by first creating a standard curve that fits all data with the specified model and then finding the concentration at a specific binding rate.
PlotBindingQuantitation -- Generates a plot of the observed binding rate as a function of concentration showing the bio-layer thickness during the quantitation step from the provided BLI analysis object.
AnalyzeEpitopeBinning -- Given a biolayer interferometry dataset, classifies antibodies by their interaction with a given antigen based on the thickness of the biolayer formed during the association of the antibodies.
PlotEpitopeBinning -- Generates a graph or table showing the groupings of samples with respect to the interactions with a particular target from a provided BLI experimental protocol.
AnalyzeDNASequencing -- Given an DNA sequencing electrophoregram fluorescence data, conducts base calling analysis which assigns DNA sequences as a function of the scan number.
PlotDNASequencing -- Generates a plot of the raw fluorescence data versus scan number from the provided DNA sequencing data.
Microscopy
AnalyzeCellCount -- Counts the apparent number of cells in the provided microscope image data.
AnalyzeColonies -- Counts the cell colonies or plaques on solid media in the provided microscope image data.
AnalyzeMicroscopeOverlay -- Generates an analytical combination of different composite fluorescent images for use in co-localization analysis.
PlotMicroscopeOverlay -- Generates a combined false color image of two or more microscope images taken with different filters from the provided microscope overlay analysis object.
PlotCellCount -- Generates an image of the microscope data with the putative cell areas indicated from the provided cell counting image analysis highlighted.
PlotCellCountSummary -- Generates an image of the microscope data with the putative cell areas highlighted, along with a pie cart of relative areas of cell groupings from the provided cell counting image analysis highlighted.
CombineFluorescentImages -- Generates a false color RGB image of the provided composite fluorescent data.
PlotImage -- Given an image file or data objects containing images, provides a visualization of these images that includes a measurement of absolute size scales when possible.
AnalyzeImageExposure -- Identify image with optimal exposure time from the provided image data.
ImageMask -- Replaces all the pixels in the input image that are outside of a given color range.
ImageOverlay -- Overlays the pixel information from two images to combine them into one.
ImageIntensity -- Computes the average intensity of the pixels as a function of the vertical pixel position in the image.
Quantification
AnalyzeAbsorbanceQuantification -- Users Beer's Law to calculate the concentration of an analyte based on its extinction coefficient and absorbance spectra.
PlotAbsorbanceQuantification -- Generates a plot of overlaid absorbance spectroscopy data from replicates reads recorded as part of the given absorbance quantification protocol.
AnalyzeTotalProteinQuantification -- Calculates total protein calculation based on total protein quantification assay data.
AnalyzeCopyNumber -- Calculates the estimated copy number of a given gene sequences in an analyte based on qPCR data or Quantification Cycle analysis.
PlotCopyNumber -- Generates a line plot of the fitted standard curve relating quantification cycle (Cq) to Log10[copy number] from the provided copy number analysis object.
AnalyzeQuantificationCycle -- Calculates the Quantification Cycle (Cq) which is the inflection point in the amplification curve of qPCR data.
PlotQuantificationCycle -- Generates a list plot of fluorescence intensity as a function of replication cycle with the quantification cycle (Cq) interpolated and highlighted from the provided quantification cycle analysis object.
AnalyzePeaks -- Preforms peak picking analysis of experimental source data connecting the results of the analysis to its source data and experimental protocol information.
AnalyzeBindingQuantitation -- Determines the concentration of the analyte measured by first creating a standard curve that fits all data with the specified model and then finding the concentration at a specific binding rate.
PlotBindingQuantitation -- Generates a plot of the observed binding rate as a function of concentration showing the bio-layer thickness during the quantitation step from the provided BLI analysis object.
Thermodynamics
AnalyzeMeltingPoint -- Calculates the melting point from a melting curve dataset (either from fluorescence or absorbance thermodynamics data).
PlotMeltingPoint -- Generates a list plot of the source melting curve analysis along with a demarcation of the fitted melting point.
AnalyzeThermodynamics -- Given a set of melting curve data or analysis, performs Van't Hoff analysis to determine free-energy, enthalpy, and entropy components of a binding event.
PlotThermodynamics -- Generates a Van't Hoff plot log of Keq as a function of inverse temperature and the fitted line containing the thermodynamic parameters from the provided thermodynamic analysis object.
AnalyzeParallelLine -- Calculates the relative potency ratio between two dose-response fitted curves.
Kinetics
AnalyzeKinetics -- Given a reaction mechanism and a set of reaction trajectories (either simulated or from experimentation) fits rate constants which represent a best fit to the trajectory data.
AnalyzeBindingKinetics -- Solves the kinetic association and dissociation rates by fitting to the source biolayer interferometry binding kinetics data.
PlotBindingKinetics -- Generates a plot comparing the prediction of fitted binding kinetics parameters to the source bio-layer interferometry data in the provided binding kinetics analysis object.
Property Measurement
AnalyzeBubbleRadius -- Computes the distribution of bubble radii from videos contained in dynamic foam analysis data.
PlotDynamicFoamAnalysis -- Generates plots of the foam height, foam volume, bubble count, mean bubble area, average bubble radius and liquid content over time from the provided dynamic foam analysis data objects.